Synthesis and assembly of hepatitis B virus surface antigen particles yeast. Nature 298, 347 (1982)


Valenzuela P., Medina A., Rutter W.J., Ammerer G. & Hall B.D.


The surface antigen of hepatitis B virus (HBsAg) has been synthesized in the yeast Saccharomyces cerevisiae by using an expression vector that employs the 5′-flanking region of yeast alcohol dehydrogenase I as a promoter to transcribe surface antigen coding sequences. The protein synthesized in yeast is assembled into particles having properties similar to the 22-nm particles secreted by human cells.


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