Currently browsing: “Recombinant chromatin”

Histone deposition and chromatin assembly by RSF

KEYWORDS:

ABSTRACT: It is becoming clear that the structure of cellular chromatin is dynamic and capable of undergoing rapid changes to respond to the metabolic requirements of the cell. These changes have a direct impact on gene expression and, therefore, the chromatin context must be considered when biochemical reactions that involve DNA are studied. Over the past several […]

Loyola A., Reinberg D. Histone deposition and chromatin assembly by RSF. Methods, vol 31: 96–103 (2003)
[↓] PDF [+] More Information

Reconstitution of recombinant chromatin establishes a requirement for histone-tail modifications during chromatin assembly and transcription

KEYWORDS:

ABSTRACT: The human ISWI-containing factor RSF (remodeling and spacing factor) was found to mediate nucleosome deposition and, in the presence of ATP, generate regularly spaced nucleosome arrays. Using this system, recombinant chromatin was reconstituted with bacterially produced histones. Acetylation of the histone tails was found to play an important role in establishing regularly spaced nucleosome arrays. Recombinant chromatin lacking […]

Loyola A., LeRoy G., Wang Y-H., Reinberg D. Reconstitution of recombinant chromatin establishes a requirement for histone-tail modifications during chromatin assembly and transcription. Genes Dev. 15(21): 2837–2851 (2001)
[↓] PDF [+] More Information