Currently browsing: “Purification”

Histone deposition and chromatin assembly by RSF

KEYWORDS: Alejandra Loyola • Chromatin Assembly • DNA • H2A • H2B • H3 • H4 • Histone deposition • Histones • Purification • Recombinant chromatin • Remodeling and spacing factor (RSF)

ABSTRACT: It is becoming clear that the structure of cellular chromatin is dynamic and capable of undergoing rapid changes to respond to the metabolic requirements of the cell. These changes have a direct impact on gene expression and, therefore, the chromatin context must be considered when biochemical reactions that involve DNA are studied. Over the past several […]

Purification and characterization of a human factor that assembles and remodels chromatin

KEYWORDS: Alejandra Loyola • Assembles • ATP-dependent • Characterization • chromatin • Chromatin Assembly • hSNF2h • Human Factor • nucleosome • Purification • Remodels

ABSTRACT: We have previously reported the isolation and characterization of a nucleosome remodeling and spacing factor, RSF. One of the RSF subunits is hSNF2h, a SNF2 homologue. Here we set out to isolate and characterize other hSNF2h-containing complexes. We have identified a novel hSNF2h complex that facilitates ATP-dependent chromatin assembly with the histone chaperone NAP-1. The complex possesses ATPase activity that is […]