Currently browsing: “nucleosome”

The Constantly Changing Face of Chromatin

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ABSTRACT: Many recent findings have altered our vision of chromatin and its role in the regulation of cellular functions. Our perspective concerning chromatin has changed to a much more complex, but also more dynamic, view of chromatin as an entity that is intimately involved in the regulation of a variety of cellular functions. In this review, we describe the various types of […]

Vaquero A., Loyola A., Reinberg D. The constantly changing face of chromatin. Sci. Aging Knowl. Environ. 2003(14): 01-16 (2003)
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Functional Analysis of the Subunits of the Chromatin Assembly Factor RSF

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ABSTRACT: The human ISWI-containing factor RSF (for remodeling and spacing factor) is composed of two subunits: the ATPase hSNF2H and p325 (Rsf-1), a protein encoded by a novel human gene. We previously showed that RSF mediates nucleosome deposition and generates regularly spaced nucleosome arrays. Here we report the characterization of the largest subunit of RSF, Rsf-1. We found […]

Loyola A., Huang J. Y., LeRoy G., Hu S., Wang Y-H., Donnelly R. J., Lane W. S., Lee S-C., Reinberg D. Functional analysis of the subunits of the chromatin assembly factor RSF. Mol. Cell. Biol. 23(19): 6759–6768 (2003)
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Purification and characterization of a human factor that assembles and remodels chromatin

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ABSTRACT: We have previously reported the isolation and characterization of a nucleosome remodeling and spacing factor, RSF. One of the RSF subunits is hSNF2h, a SNF2 homologue. Here we set out to isolate and characterize other hSNF2h-containing complexes. We have identified a novel hSNF2h complex that facilitates ATP-dependent chromatin assembly with the histone chaperone NAP-1. The complex possesses ATPase activity that is […]

LeRoy G., Loyola A., Lane W. S., Reinberg D. Purification and characterization of a human factor that assembles and remodels chromatin. J. Biol. Chem. 275(20): 14787–14790 (2000)
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