Currently browsing: “Nap-1”

Histone chaperones, a supporting role in the limelight

KEYWORDS:

ABSTRACT: In eukaryotic cells, highly basic histone proteins are associated with the DNA to form the nucleosome, the fundamental unit of chromatin. Histones are closely escorted by histone chaperones from their point of synthesis up to their delivery site. We will present an overview of the histone chaperones identified to date with their various roles, in an […]

Loyola A., Almouzni G. Histone chaperones, a supporting role in the limelight. Institut Curie/Section de Recherche 1677, 03-11 (2004)
[↓] PDF [+] More Information

Reconstitution of recombinant chromatin establishes a requirement for histone-tail modifications during chromatin assembly and transcription

KEYWORDS:

ABSTRACT: The human ISWI-containing factor RSF (remodeling and spacing factor) was found to mediate nucleosome deposition and, in the presence of ATP, generate regularly spaced nucleosome arrays. Using this system, recombinant chromatin was reconstituted with bacterially produced histones. Acetylation of the histone tails was found to play an important role in establishing regularly spaced nucleosome arrays. Recombinant chromatin lacking […]

Loyola A., LeRoy G., Wang Y-H., Reinberg D. Reconstitution of recombinant chromatin establishes a requirement for histone-tail modifications during chromatin assembly and transcription. Genes Dev. 15(21): 2837–2851 (2001)
[↓] PDF [+] More Information