Currently browsing: “ISWI-containing”

Functional Analysis of the Subunits of the Chromatin Assembly Factor RSF

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ABSTRACT: The human ISWI-containing factor RSF (for remodeling and spacing factor) is composed of two subunits: the ATPase hSNF2H and p325 (Rsf-1), a protein encoded by a novel human gene. We previously showed that RSF mediates nucleosome deposition and generates regularly spaced nucleosome arrays. Here we report the characterization of the largest subunit of RSF, Rsf-1. We found […]

Loyola A., Huang J. Y., LeRoy G., Hu S., Wang Y-H., Donnelly R. J., Lane W. S., Lee S-C., Reinberg D. Functional analysis of the subunits of the chromatin assembly factor RSF. Mol. Cell. Biol. 23(19): 6759–6768 (2003)
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Reconstitution of recombinant chromatin establishes a requirement for histone-tail modifications during chromatin assembly and transcription

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ABSTRACT: The human ISWI-containing factor RSF (remodeling and spacing factor) was found to mediate nucleosome deposition and, in the presence of ATP, generate regularly spaced nucleosome arrays. Using this system, recombinant chromatin was reconstituted with bacterially produced histones. Acetylation of the histone tails was found to play an important role in establishing regularly spaced nucleosome arrays. Recombinant chromatin lacking […]

Loyola A., LeRoy G., Wang Y-H., Reinberg D. Reconstitution of recombinant chromatin establishes a requirement for histone-tail modifications during chromatin assembly and transcription. Genes Dev. 15(21): 2837–2851 (2001)
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