Currently browsing: “H2A”

The Constantly Changing Face of Chromatin

KEYWORDS: advancements • Alejandra Loyola • ATP-dependent • chromatin • DNA • H2A • Histone Variants • nucleosome • Nucleosome Structure: • proteins

ABSTRACT: Many recent findings have altered our vision of chromatin and its role in the regulation of cellular functions. Our perspective concerning chromatin has changed to a much more complex, but also more dynamic, view of chromatin as an entity that is intimately involved in the regulation of a variety of cellular functions. In this review, we describe the various types of […]

Histone deposition and chromatin assembly by RSF

KEYWORDS: Alejandra Loyola • Chromatin Assembly • DNA • H2A • H2B • H3 • H4 • Histone deposition • Histones • Purification • Recombinant chromatin • Remodeling and spacing factor (RSF)

ABSTRACT: It is becoming clear that the structure of cellular chromatin is dynamic and capable of undergoing rapid changes to respond to the metabolic requirements of the cell. These changes have a direct impact on gene expression and, therefore, the chromatin context must be considered when biochemical reactions that involve DNA are studied. Over the past several […]

Facile synthesis of site-specifically acetylated and methylated histone proteins: Reagents for evaluation of the histone code hypothesis

KEYWORDS: acetylation • Alejandra Loyola • genetically encoded • H2A • H2B • H3 • H4 • histone code • histone proteins • methylation modifications • peptide synthesis

ABSTRACT: The functional capacity of genetically encoded histone proteins can be powerfully expanded by posttranslational modification. A growing body of biochemical and genetic evidence clearly links the unique combinatorial patterning of side chain acetylation, methylation, and phosphorylation mainly within the highly conserved N termini of histones H2A, H2B, H3, and H4 with the regulation of gene expression and chromatin assembly […]