Currently browsing: “chromatin”

The Constantly Changing Face of Chromatin

KEYWORDS: advancements • Alejandra Loyola • ATP-dependent • chromatin • DNA • H2A • Histone Variants • nucleosome • Nucleosome Structure: • proteins

ABSTRACT: Many recent findings have altered our vision of chromatin and its role in the regulation of cellular functions. Our perspective concerning chromatin has changed to a much more complex, but also more dynamic, view of chromatin as an entity that is intimately involved in the regulation of a variety of cellular functions. In this review, we describe the various types of […]

Purification and characterization of a human factor that assembles and remodels chromatin

KEYWORDS: Alejandra Loyola • Assembles • ATP-dependent • Characterization • chromatin • Chromatin Assembly • hSNF2h • Human Factor • nucleosome • Purification • Remodels

ABSTRACT: We have previously reported the isolation and characterization of a nucleosome remodeling and spacing factor, RSF. One of the RSF subunits is hSNF2h, a SNF2 homologue. Here we set out to isolate and characterize other hSNF2h-containing complexes. We have identified a novel hSNF2h complex that facilitates ATP-dependent chromatin assembly with the histone chaperone NAP-1. The complex possesses ATPase activity that is […]