Researh & development

Organelle Phagy Lab in Health & Disease


Cells continually degrade molecules and organelles, processes that are increased in response to stress conditions as a survival mechanism. Autophagy is one of these processes characterized by depending on the degradative function of lysosomes, membranous organelles rich in acidic hydrolases. Lysosomes degrade molecules and organelles that are detected and captured by “sub-cellular sentinels” called autophagosomes (macroautophagy) and multivesicular bodies (microautophagy) Autophagy is also closely interconnected with the function of the proteasome and ERAD pathway at the endoplasmic reticulum, processes dependent on the ubiquitin molecule. Our group studies the cellular and molecular mechanisms of these degradative processes in an integrative manner, incorporating different stressful scenarios such as chronic inflammation, hypoxia and chemotherapeutic drugs. In this context, we study the role of HERPUD1, Galectin-3 and Amyloid Precursor Protein, applying a basic and clinical approach.

  • Research Head: Patricia Burgos
  • Doctoral Students: Nicolás Albornoz, Laura Hernández, Javiera Álvarez, Jorge Catalán
  • Master Student: Francisca Cruzat
  • Research Assistants: Omar Cortés, Eloisa Arias
  • Post-doctoral Fellows: Viviana Cavieres, Cristóbal Troncoso

  • Hianara Bustamante (Former undergraduate student and Ph.D. student, 2013- 2018). Assistant professor, Universidad Austral de Chile, Chile
  • Claudia Yefi (Former Ph.D. student, 2011-2016). Assistant professor, P. Universidad Católica de Chile, Chile
  • Alexis González (Former Ph.D. student, 2011-2016). Postdoctoral Fellow, Goethe University Frankfurt, Frankfurt, Germany
  • Gabriela Vargas (Former Ph.D. student, 2017-2022). Postdoctoral Fellow, P. Universidad Católica de Chile, Chile
  • Andrés Rivera-Dictter Q.E.P.D. (Former undergraduate student 2010-2012)
  • Vanessa Muñoz (Former undergraduate student 2011-2013)
  • Rafael Barraza (Former undergraduate student 2014-2016)
  • Gustavo Leal (Former undergraduate student 2014-2016)
  • Yorka Cheuquemilla (Former undergraduate student 2015-2017)
  • Susanne Campos (Former undergraduate student 2015-2017)
  • Jorge Catalán (Former undergraduate student 2017-2018)
  • Camila Concha (Former undergraduate student 2021)

National Collaborations

Pamela Ehrenfeld (UACh)

  • María José Barrera (USS)
  • Manuel Varas-Godoy (USS)
  • Eugenia Morselli (USS)
  • Isabel Saffie (FALP)
  • Gonzalo Mardones (UACh)
  • Bredford Kerr (USS).

Internationals Collaborations

  • Luis DaSilva (Sao Paulo University)
  • Stephannie Misserey-Lenkei (Curie Institute)
  • Li Yu (Tsinghua University)


Burgos, P., Metz, C., Bull, P., Pincheira, R., Massardo, L., Errázuriz, C., Bono, M.R., Jacobelli, S., and González, A. (2000) Increased expression of c-rel, from the NF-B/Rel family, in T cells from patients with systemic lupus erythematosus. J. Rheumatol. 27: 116-27.

Massardo, L., Burgos, P., Martínez, M.E., Pérez, R., Calvo, M., Barros, J., González, A., and Jacobelli, J. (2002) Antiribosomal P protein antibodies in chilean SLE patients: no association with renal disease. Lupus 11: 379-383.

Burgos P.V., Klattenhoff C., de la Fuente E., Rigotti A., González, A. (2004)Cholesterol depletion induces PKA-mediated basolateral-to-apical transcytosis of the scavenger receptor class B type I in MDCK cells. Proc. Natl. Acad. Sci. USA. 101 (11): 3845-50.

Wasmeier, C., Burgos, P.V., Trudeau T, Davidson H.W., and Hutton J.C. (2005)An extended tyrosine-targeting motif for endocytosis and recycling of the dense-core vesicle membrane protein phogrin. Traffic 6: 474-487.

Kametaka S., Moriyama K., Burgos P.V., Eisenberg E., Greene L., Mattera R., and Bonifacino J.S. (2007) Canonical interaction of cyclin G-associated kinase with adaptor protein 1 regulates lysosomal enzyme sorting. Mol. Biol. Cell. 18 (8): 2991-3001.

*Mardones G.A., *Burgos P.V., Brooks D.A., Parkinson-Lawrence E., Mattera R., and Bonifacino J.S. (2007) The trans-Golgi network accessory protein p56 promotes long-range movement of GGA/clathrin-containing transport carriers and lysosomal enzyme sorting. Mol. Biol. Cell. 18 (9): 3486-501. * authors with equal contribution

*Matus S., *Burgos P.V., Bravo-Zehnder M., Kraft R., Porras O.H., Farías P., Barros L.F., Torrealba F., Massardo L., Jacobelli, S., González A. (2007) Antiribosomal-P autoantibodies from psychiatric lupus target a novel neuronal surface protein causing calcium influx and apoptosis. J. Exp. Med. 204 (13): 3221-34. * Authors with equal contribution

DaSilva L., Sougrat R., Burgos, P.V., Janvier K., and Bonifacino J.S. (2009) The human immunodeficiency virus-1 Nef protein mediates ubiquitination-independent targeting of CD4 co-receptor to the multivesicular body pathway. J. Virol. 83 (13): 6578-90. PMC2698520

Popoff V., Mardones G.A., Bai S.K., Chambon V., Tenza D., Burgos P.V., Shi A., Benaroch P., Urbé S., Lamaze C., Grand B.D., Raposo G., and Johannes L. (2009) Analysis of articulation between clathrin and retromer in retrograde sorting on early endosomes. Traffic 10 (12): 1868-80.

Burgos, P.V, Mardones, G.A., Rojas, A., daSilva, L., Prabhu, Y., Hurley, J.H., and Bonifacino, J.S. (2010) Sorting of the Alzheimer’s disease amyloid precursor protein mediated by AP-4 complex. Dev. Cell 18 (3): 425-36.

Shiba Y., Römer W., Mardones G.A., Burgos P.V., Lamaze C., Johannes L. (2010) AGAP2 regulates retrograde transport between early endosomes and the TGN. J Cell Sci 123(14): 2381-90. PMC2894655

Im Y.J., Kuo L., Ren X., Burgos P.V., Zhao X.Z., Liu F., Burke T.R. Jr., Bonifacino J.S., Freed E.O., Hurley J.H. (2010) Crystallographic and functional analysis of the ESCRT-I /HIV-1 Gag PTAP interaction. Structure 18(11):1536-47.

Inestrosa N.C., Tapia-Rojas C., Griffith T. N., Carvajal F. J., Benito M. J., Rivera-Dictter A., Alvarez A.R., Serrano F.G., Hancke J.L., Burgos P.V., Parodi J., and Varela-Nallar L. (2011) Tetrahydrohyperforin prevents cognitive deficit, Aβ deposition, tau phosphorylation and synaptotoxicity in the APPswe/PSEN1ΔE9 model of Alzheimer’s disease: a possible effect on APP processing. Translational Psychiatry 1: e20; doi:10.1038/tp.2011.19.

Prabhu, Y., Burgos P.V., Schindler, C., Farías, G.G., Magadán JG, and Bonifacino J.S. (2012) Adaptor protein 2-mediated endocytosis of the b secretase BACE1 is dispensable for amyloid precursor protein processing. Mol. Biol. Cell. 23 (12): 2339-51.

Mardones G.A., Burgos P.V., Lin Y., Kloer D.P., Magadán J.G., Hurley J.H., Bonifacino J.S. (2013) Structural Basis for the Recognition of Tyrosine-based Sorting Signals by the μ3A Subunit of the AP-3 Adaptor Complex. J. Biol. Chem. 288 (13): 9563-71

Bustamante H.A., Rivera-Dictter A., Cavieres V.A., Muñoz V., González A., Lin Y., Mardones G.A., and Burgos P.V.* (2013) Turnover of C99 is controlled by a crosstalk between ERAD and ubiquitin-independent lysosomal degradation in human neuroglioma cells. PLoS One Dec 20;8(12):e83096.

Montes D.K., Brenet M., Muñoz V.C., Burgos P.V., Villanueva C.I., Figueroa C.D., González C.B. (2013) Vasopressin activates Akt/mTOR pathway in smooth muscle cells cultured in high glucose concentration. Biochem Biophys Res Commun 441:923-928

Ross B.H., Lin Y., Corales E.A., Burgos P.V., and Mardones G.A. (2014) Structural and Functional Characterization of Cargo-Binding Sites on the 4-Subunit of Adaptor Protein Complex 4. PLoS One Feb 3;9(2):e88147

Viviana A. Cavieres; Alexis González; Vanessa C. Muñoz, Claudia P. Yefi, Hianara A. Bustamante, Rafael R. Barraza, Cheril Tapia-Rojas, Carola Otth, María José Barrera, Carlos González, Gonzalo A. Mardones, Nibaldo C. Inestrosa and Patricia V. Burgos* (2015) Tetrahydrohyperforin inhibits the proteolytic processing of amyloid precursor protein and enhances its degradation by Atg5-dependent autophagy. PLoS One Aug 26;10(8):e0136313

Alexis González; Viviana A. Cavieres; Nibaldo Inestrosa; Patricia V. Burgos* (2016) Tetrahydrohyperforin (IDN5706) targets the ER for autophagy activation: potential mechanism for Alzheimer´s disease therapy. Neural Regeneration Research Feb;11(2):242-243

LF Bátiz, M Castro, P.V. Burgos, Z Velasquez, P Troncoso, C Lafourcade, RI Muñoz, Ursula Wyneken (2016). Role of extracellular vesicles and the vascular niche on neuronal stem cells and adult neurogenesis. Frontiers in Cellular Neuroscience Jan 19;9:501

Cheril Tapia-Rojas, Patricia V. Burgos* and Nibaldo Inestrosa (2016)Inhibition of Wnt Signalling induces amyloidogenic processing of APP, production and aggregation of 1-42 amyloid-b peptides. J. Neurochemistry doi: 10.1111/jnc. 13873

Maria Jose Yañez, Olivia Belbin, Lisbell Estrada, Nancy Leal, Pablo Contreras, Alberto Lleó, Patricia V. Burgos, Silvana Zanlungo, and Alejandra Alvarez. c-Abl links APP-BACE1 interaction promoting APP amyloidogenic processing in Niemann-Pick type C Disease (2016) Biochimica et Biophysica Acta (BBA) 1862 (11): 2158

María José Tenorio; Breyan H. Ross; Charlotte Luchsinger; Cecilia Arriagada; Diego Acuña; Andrés Rivera-Dictter; Marcelo Aguilar; Viviana Cavieres; Pamela Ehrenfeld; Patricia V. Burgos; Gonzalo A. Mardones (2016). .Distinct biochemical pools of Golgi phosphoprotein 3 in the human breast cancer cell lines MCF7 and MDA-MB-231. PLoS One Apr 28; 11(4)

Alexis González, Vanessa Muñoz, Viviana Cavieres, Hianara Bustamante, Victor Cornejo, Yunan C. Januario, Ibeth González, Claudio Hetz, Luis Da Silva, Alejandro Rojas-Fenandez, Ronald Hay, Gonzalo A. Mardones and Patricia V. Burgos*** (2017) Autophagosomes cooperate in the degradation of intracellular C-terminal fragments of the Amyloid Precursor Protein via the MVB/lysosomal pathway. FASEB Journal, March 2 doi: 10.1096/fj.201600713R

Carolina Martin, Luis Leyton, Melissa Hott, Yennyfer Arancibia, Carlos Spichiger, Mark McNiven, Felipe A. Court, Margarita I. Concha, Patricia V. Burgos, Carola Otth (2017) Herpes Simplex Virus type 1 neuronal infection perturbs Golgi apparatus integrity through activation of Src tyrosine kinase and Dyn-2 GTPase. Frontiers in Cellular and Infection Microbiology, accepted. Aug 22;7:371. doi: 10.3389/fcimb.2017.00371

Covarrubias-Pinto A, Acuña AI, Boncompain G, Papic E, Burgos PV, Perez F, Castro MA. (2018) Ascorbic acid increases SVCT2 localization at the plasma membrane by accelerating its trafficking from early secretory compartments and through the endocytic-recycling pathway Free Radic Biol Med Mar 12;120:181-191. doi: 10.1016/j.freeradbiomed.2018.03.013

Luchsinger C, Aguilar M, Burgos PV, Ehrenfeld P, Mardones GA (2018) Functional disruption of the Golgi apparatus protein ARF1 sensitizes MDA-MB-231 breast cancer cells to the antitumor drugs Actinomycin D and Vinblastine through ERK and AKT signaling PLoS One. 2018 Apr 3;13(4):e0195401. doi: 10.1371/journal.pone.0195401.

Bustamante HA, González AE, Cerda-Troncoso C, Shaughnessy R, Otth C, Soza A and Burgos PV* (2018)Interplay between the autophagy-lysosomal pathway and the ubiquitin-proteasome system: a target for therapeutic development in Alzheimer’s disease Frontiers in Cellular Neurosciences May 9; 12:126

Acuña-Hinrichsen F, Muñoz M, Mancilla E, Salazar P, Burgos PV, Zambrano A, Concha MI and Otth C (2019) Herpes Simplex Virus Type 1 enhances expression of the Synaptic protein Arc for its Own Benefit. Frontiers in Cellular Neurosciences Jan 8; 12:505

Diego Tapia, Tomás Jiménez, Javier Espinoza, Constanza Zamora, Riccardo Rizzo, Alexis González-Cárdenas, Danitza Fuentes, Andrea Soza, Fanny Guzmán, Gloria Arriagada, Maria Isabel Yuseff, Gonzalo Mardones, Patricia V. Burgos, Alfonso González, Alberto Luini and Jorge Cancino. KDEL receptor regulates secretion by lysosome relocation- and autophagy-dependent modulation of lipid-droplet turnover (2018) Nat Commun. 2019 Feb 13;10(1):735.

Cecilia Arriagada, Charlotte Luchsinger, Alexis González, Tomás Schwenke, Gloria Arriagada, Hugo Folch, Pamela Ehrenfeld, Patricia V. Burgos, and Gonzalo A. Mardones. The knocking down of the oncoprotein Golgi phosphoprotein 3 in T98G cells of glioblastoma multiforme disrupts cell migration by affecting focal adhesion dynamics in a focal adhesion kinase-dependent manner (2019) PLoS One. 2019 Feb 19;14(2):e0212321.

Nicolás Albornoz, Hianara Bustamante, Andrea Soza, and Patricia V. Burgos Cellular responses to proteasome inhibition: Molecular mechanisms and Beyond (2019) Int. J. Mol. Sci. 10; 20(14)

Bustamante HA, Cereceda K, González AE, Valenzuela GE, Cheuquemilla Y, Hernández S, Arias-Muñoz E, Cerda-Troncoso C, Bandau S, Soza A, Kausel G, Kerr B, Mardones GA, Cancino J, Hay RT, Rojas-Fernandez A and Burgos PV The proteasomal Deubiquitinating enzyme PSMD14 regulates macroautophagy by controlling Golgi-to-ER retrograde transport. (2020) Cells 2020 Mar 23;9(3):777

Viviana Cavieres, Cristóbal Cerda-Troncoso, Andrés Rivera-Dictter, Rodrigo Castro, Charlotte Luchsinger, Patricia V. Burgos & Gonzalo A. Mardones. Human Golgi phosphoprotein 3 is a non-canonical effector of RAB1A and RAB1B (2020) PLoS One. 2020 Aug 13;15(8):e0237514. doi: 10.1371/journal.pone.0237514. eCollection 2020.

María Paz Hernández-Cáceres, Karina Cerceda, Sergio Hernández, Ying Li, Carla Narro, Patricia Rivera, Patricio Silva, Yenniffer Ávalos, Claudia Jara, Paulina Burgos, Lilian Toledo, Pablo Lagos, Flavia Cifuentes, Claudio Perez-Leighton, Cristina Bertocchi, Deborah Clegg, Alfredo Criollo, Cheril Tapia-Rojas, Patricia V Burgos, Eugenia Morselli. Palmitic acid reduces the autophagic flux in hypothalamic neurons by impairing autophagosome-lysosome fusion and endolysosomal dynamics (2020). Molecular & Cellular Oncology Jul 25;7(5):1789418. doi: 10.1080/23723556.2020.1789418

Cecilia Arriagada, Viviana A. Cavieres, Charlotte Luchsinger, Alexis E. González, Vanessa C. Muñoz, Jorge Cancino, Patricia V. Burgos and Gonzalo A. Mardones. GOLPH3 regulates EGFR endocytic trafficking by modulating its glycosylation and ubiquitylation in T98G glioblastoma cells (2020) Int. J. Mol. Sci. 2020 Nov 23;21(22):8880. doi: 10.3390/ijms21228880.

Cerda-Troncoso C, Varas-Godoy M, and Burgos PV. Pro-tumoral functions of autophagy receptors in the modulation of cancer progression (2021). Frontiers in Oncology doi: 10.3389/fonc.2020.619727

Yunan C Januário, Luan S de Oliveira, Raffaella De Pace, Patricia V Burgos, David Gershlick, Sonia AL Correa, Luis LP daSilva. AP-1 mediated Golgi export of the amyloid precursor protein prevents the intracellular accumulation of its neurotoxic fragment C99 (2022) J Biol Chem. 2022 Jun 23:102172. doi: 10.1016/j.jbc.2022.102172. Online ahead of print.

Vargas G, Cortés O, Arias-Muñoz E, Hernández S, Cerda-Troncoso C, Tatham M, Bustamante H, Cancino J, Mardones GA, Rojas-Fernandez A, Varas-Godoy M, Cavieres VA and Burgos PV. Negative modulation of macroautophagy by stabilized HERPUD1 is counteracted by an increased ER-lysosomal Network with impact in drug-induced stress cell survival (2022) Frontiers in Cell and Developmental Biology March Volumen 10, Article 743287 doi: 10.3389/fcell.2022.743287

Rodrigo Espinosa, Karla Gutiérrez, Javiera Rios, Fernando Ormeño, Liliana Yantén, Pablo Galaz-Davison, César A. Ramírez-Sarmiento, Valentina Parra, Amelina Albornoz, Iván E. Alfaro, Patricia V. Burgos, Eugenia Morselli, Alfredo Criollo and Mauricio Budini Palmitic and Stearic Acids Inhibits Chaperone-mediated Autophagy (CMA) in POMC-like Neurons In Vitro (2022) Cells, 11, 920

Nohela B. Arévalo, Cristian M. Lamaizon, Viviana A. Cavieres, Patricia V. Burgos, Alejandra Rojas Álvarez, María José Yañez and Silvana Zanlungo. Neuronopathic Gaucher Disease: beyond lysosomal dysfunction (2022) Frontiers in Molecular Neuroscience (accepted)

Daniel Peña-Oyarzún, Carla San Martin, María Paz Hernández-Cáceres, Sergio Lavandero, Eugenia Morselli, Mauricio Budini, Patricia V. Burgos, and Alfredo Criollo. Autophagy in aging-related oral diseases (2022) Frontiers in Endocrinology (accepted).

Yenniffer Ávalos, María Paz Hernández-Cáceres, Pablo Lagos, Daniela Pinto Nuñez, Patricia Rivera, Paulina Burgos, Francisco Díaz-Castro, Michelle Joy Immediato, Ana Batista, Flavia Cifuentes-Araneda, Lilian Toledo-Valenzuela, Marcelo Rodriguez, Jasson Espinoza, Claudio Perez-Leighton, Cristina Bertocchi, Rodrigo Troncoso, Valentina Parra, Mauricio Budini, Patricia V. Burgos, Alfredo Criollo*, Eugenia Morselli*. Palmitic acid control of ciliogenesis modulates insulin signaling in hypothalamic neurons through an autophagy-dependent mechanism (2022). Cell Death and Diseases (accepted).

Principal investigator

Patricia Burgos