Molecular Virology

PROFILE

Viruses infect all domains of life and have an important role in public health and evolution. We follow basic questions related to the viral biology of infection, with special focus on the envelope proteins of viruses, which they use to enter and exit cells. Therefore, we use as model systems the human pathogenic hantaviruses and also membrane-containing archaeal viruses. For hantaviruses we have provided molecular insights into its virus:cell membrane fusion mechanism and characterized the organization and dynamics of the fusion protein Gc on the viral surface. For the design of vaccine and drug candidates we also focus on protective immune responses against hantavirus, using non-replicative virus-like particles developed previously by us. Based on our experience with the fusion protein of animal-infecting hantaviruses, we have identified the fusion activity of enveloped halophilic archaeal viruses (pleolipoviruses). We expect that future mechanistic information will allow us to unveil how viruses modulate archaeal membranes in this yet poorly explored life domain.

LAB MEMBERS

Research Head: Nicole Tischler

Doctoral Students: Fabian Figueroa

Undergraduate Students: Gianina Alessan • Kevin Chou • Francisca Cruzat • Esteban Rodriguez

Research Assistants: Diego González-Candia

Post-doctoral Fellows: Eduardo Bignon • Nicolás Muena

Research Associate: Amelina Albornoz

COLLABORATIONS

National Collaborations
• Dr. Alejandro Rojas, Universidad Austral, Chile
• Dr. Fernando Torrés-Perez, Universidad Católica de Valparaiso, Chile
• Dr. Marcela Ferrés, Pontificia Universidad Católica de Chile
• Dr. Marcelo López-Lastra & Dr Jorge Vera-Otarola, Pontificia Universidad Católica de Chile
• Dr. Rafael Medina, Pontificia Universidad Católica de Chile

International Collaborations
• Dr. Elina Roine; Dr Juha Huiskonen, Helsinki University, Finland
• Dr. Félix Rey & Dr Pablo Guardado-Calvo, Pasteur Institut, France
• Dr. Jay Hooper, USAMRIID, USA
• Dr. Juan Reguera, AFMB, Aix-Marseille University/CNRS, France
• Dr. Kartik Chandran & Dr Rohit Jangra, Albert Einstein College of Medicine, USA
• Dr. Pierre-Yves Lozach, University Hospital Heidelberg, Germany; University Claude Bernard, France
• Dr. Salvatore Chiantia, Potsdam Universität, Germany
• Dr. Susanne Erdmann, Max Planck Institut Bremen, Germany

FORMER LAB MEMBERS (selection)

Former PhD studentes:
• Nicolás Cifuentes-Muñoz (2006-2011). Assistant Professor at Universidad Autónoma de Chile
• Gonzalo Barriga (2009-2014). Assistant Professor at University of Chile
• Rodrigo Acuña (2009-2015). Teaching Researcher, Universidad del Desarrollo de Chile
• María Pía García (2012-2019). Regulatory affairs exports, Drag Pharma Laboratory Invetec S.A., Chile

Former Master & undergraduate students:
• Simón Vidal (2008-2009). Post-doctoral researcher at Genentech, USA
• Ana Elola (2012-2014). Director of scientific education, Co-founder of BioClass, Chile
• Fernando Villalón (2010–2011 & 2011-2014 RA). PhD student, University of Melbourne, Australia
• Anke Roder, (2009-2010). Research Specialist at Procter & Gamble, Germany
• Chantal Márquez (2012-2014). Post-doctoral researcher, Universidad de Chile
• Natalia Salazar-Quiroz (2012-2014). PhD student, University of Melbourne, Australia
• Vicente Muñoz (2014-2016). PhD student, Universidad de Chile

SELECTED PUBLICATIONS

  • Serris A, Stass R, Bignon EA, Muena NA, Manuguerra JC, Jangra RK, Li S, Chandran K, Tischler ND, Huiskonen JT, Rey FA, Guardado-Calvo P. (2020) High-Resolution Description of the Hantavirus Surface Glycoprotein Lattice and Its Membrane Fusion Control Mechanism. Cell 183:1-15 doi: 10.1016/j.cell.2020.08.023 | PDF | ABSTRACT
  • Ferrés M*, Martínez-Valdebenito C, Angulo J, Henríquez C, Vera-Otárola J, Vergara MJ, Pérez J, Fernández J, Sotomayor V, Valdés MF, González-Candia D, Tischler ND, Vial C, Vial P, Mertz G, Le Corre N* (2020). Mother-to-Child Transmission of Andes Virus through Breast Milk. Emerg Infect Dis 26(8):1885-1888. doi: 10.3201/eid2608.200204. | PDF | ABSTRACT
  • Otarola JV*, Solis L, Lowy F, Olguín V, Angulo J, Pino K, Tischler ND, Otth C, Padula P, López-Lastra M* (2020). The Andes orthohantavirus NSs protein antagonizes the type I interferon response by inhibiting MAVS signaling. J Virol. 00454-202020 doi: 10.1128/JVI.00454-20. | PDF | ABSTRACT
  • Clement J*, Ahlm C, Avšič-Županc T, Botten J, Chandran K, Jonsson CB, Kariwa H, Klingström J, Klempa B, Krüger DH, Leirs H, Li D, Liang M, Markotić A, Papa A, Schmaljohn CS, Tischler ND, Ulrich RG, Vaheri A, Vial C, Yanagihara R, Maes P. (2020) Meeting report: Eleventh International Conference on Hantaviruses. Antiviral Res. 176:104733. doi: 10.1016/j.antiviral.2020.104733. | PDF | ABSTRACT
  • Macromolecule Particle Picking and Segmentation of a KLH Database by Unsupervised Cryo-EM Image Processing. Carrasco M*, Toledo P, Tischler ND. (2019) Biomolecules 9(12). pii: E809. doi: 10.3390/biom9120809. | PDF | ABSTRACT
  • Uckeley ZM, Koch J, Tischler ND, Léger P, Lozach PY*. Cell biology of phlebovirus entry. Virologie (Montrouge). 2019 Jun 1;23(3):176-187. doi: 10.1684/vir.2019.0780. | PDF | ABSTRACT
  • Bignon, EA, Albornoz, A, Guardado-Calvo, P, Rey, FA*, Tischler, ND* (2019). Molecular organization and dynamics of the fusion protein Gc at the hantavirus surface. eLife e46028. doi: 10.7554/eLife.46028. | PDF | ABSTRACT
  • El Omari, K, Li, S, Kotecha, A, Walter, TS; Bignon EA; Harlos, K; Somerharju, P; De Haas, F; Clare DK; Molin, M; Hurtado, F; Li, M; Grimes, JM; Bamford, DH; Tischler, ND*; Huiskonen, JT*; Stuart, DI*; Roine, E.* (2019). The structure of a prokaryotic viral envelope protein expands the landscape of membrane fusion proteins. Nature Com, 10(1):846. doi: 10.1038/s41467-019-08728-7. | PDF | ABSTRACT
  • Jangra, RK; Herbert, AS; Li, R.; Jae, LT; Kleinfelter, LM; Slough, MM; Barker, SL; Guardado-Calvo, P; Román-Sosa, R; Dieterle, ME; Kuehne, AI; Muena, NA; Wirchnianski, AS; Nyakatura, EK; Fels, JM; Ng, M; Mittler, E; Pan, J.; Bharrhan, S; Wec, AZ; Lai, JR; Sidhu, SS; Tischler, ND; Rey, FA; Moffat, J; Brummelkamp, TR*; Wang, Z*; Dye, JM*; Chandran, K* (2018). Protocadherin-1 is critical for cell entry by New-World hantaviruses. Nature 563(7732):559-563. doi: 10.1038/s41586-018-0702-1. | PDF | ABSTRACT
  • Guardado-Calvo, P, Bignon, EA, Stettner, E, Jeffers SA, Pehau-Arnaudet, G, Tortorici, MA, Jestin, JL, England, P, Tischler, ND*, Rey, FA* (2016). Mechanistic insight into bunyavirus-induced membrane 1 fusion from structure-function analyses of the hantavirus envelope glycoprotein Gc. PLoS Path 12(10):e1005813. doi: 10.1371/journal.ppat.1005813. | PDF | ABSTRACT
  • Willensky, S, Bar-Rogovsky, H, Bignon, EA, Tischler, ND, Modis, Y, Dessau, M* (2016). Crystal Structure of Glycoprotein C from a Hantavirus in the Post-fusion Conformation. PLoS Path 12(10):e1005948. doi: 10.1371/journal.ppat.1005948. | PDF | ABSTRACT
  • Albornoz A, Hoffmann AB, Lozach PY, Tischler ND (2016). Early Bunyavirus-host cell interactions. Viruses 24;8(5). doi: 10.3390/v8050143. | PDF | ABSTRACT
  • Barriga GP, Villalón-Letelier F, Márquez CL, Bignon EA, Acuña R, Ross BH, Monasterio O, Mardones GA, Vidal SE, Tischler ND (2016). Inhibition of the hantavirus fusion process by predicted domain III and stem peptides from glycoprotein Gc. PLoS Negl Trop Dis 10(7), e0004799. doi: 10.1371/journal.pntd.0004799 | PDF | ABSTRACT
  • Acuña R., Bignon E.A., Mancini R., Lozach P.Y., Tischler N.D. (2015). Acidification triggers Andes hantavirus membrane fusion and rearrangement of Gc into a stable post-fusion homotrimer. J Gen Virol. 96(11):3192-7. doi: 10.1099/jgv.0.000269 | PDF | ABSTRACT
  • Acuña R, Cifuentes-Muñoz N, Márquez CL, Bulling M, Klingström J, Mancini R, Lozach PY, Tischler, ND (2014). Hantavirus Gn and Gc glycoproteins self-assemble into virus-like particles . Journal of Virology, vol. 88(4):2344-2348. doi: 10.1099/jgv.0.000269 | PDF | ABSTRACT
  • Cifuentes-Muñoz N, Salazar-Quiroz N, Tischler ND (2014). Hantavirus Gn and Gc envelope glycoproteins: structural key units for virus cell entry and virus assembly. Viruses, vol. 21,6(4):1801–22 doi: 10.3390/v6041801 | PDF | ABSTRACT