Currently browsing: “Publications”

Selective imprinting of gut-homing T cells by Peyer’s patch dendritic cells

ABSTRACT: Whereas naive T cells migrate only to secondary lymphoid organs¹˒², activation by antigen confers to T cells the ability to home to non-lymphoid sites³˒⁴. Activated effector/memory T cells migrate preferentially to tissues that are connected to the secondary lymphoid organs where antigen was first encountered⁵⁻⁷. Thus, oral antigens induce effector/memory cells that express essential […]

Mora J. R., Bono M. R., Manjunath N., Weninger W., Cavanagh L. L., Rosemblatt M. and Von Andrian U. H. Selective imprinting of gut-homing T cells by Peyer’s patch dendritic cells. Nature 424, 88-93 (2003)
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Structural Features Underlying the Multisite Phosphorylation Of the A Domain of the NF-AT4 Transcription Factor by Protein Kinase CK1.

ABSTRACT: The phosphorylation and dephosphorylation of the NF-AT family of transcription factors play a key role in the activation of T lymphocytes and in the control of the immune response. The mechanistic aspects of NF-AT4 phosphorylation by protein kinase CK1 have been studied in this work with the aid of a series of 27 peptides, […]

Marin O., Burzio V., Boschetti M., Meggio F., Allende C. C., Allende J. E. and Pinna L. A. Structural Features Underlying the Multisite Phosphorylation of the A Domain of the NF-AT4 Transcription Factor by Protein Kinase CK1. Biochemistry 41, 618-627 (2002)
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Reconstitution of recombinant chromatin establishes a requirement for histone-tail modifications during chromatin assembly and transcription

ABSTRACT: The human ISWI-containing factor RSF (remodeling and spacing factor) was found to mediate nucleosome deposition and, in the presence of ATP, generate regularly spaced nucleosome arrays. Using this system, recombinant chromatin was reconstituted with bacterially produced histones. Acetylation of the histone tails was found to play an important role in establishing regularly spaced nucleosome arrays. Recombinant chromatin lacking […]

Loyola A., LeRoy G., Wang Y-H., Reinberg D. Reconstitution of recombinant chromatin establishes a requirement for histone-tail modifications during chromatin assembly and transcription. Genes Dev. 15(21): 2837–2851 (2001)
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The genomic structure of two Protein Kinase CK2 alpha genes of Xenopus laevis and features of the putative promoter region

ABSTRACT: Protein kinase CK2 is an enzyme that is ubiquitous in eukaryotes. This enzyme, composed of catalytic (α and α′) and regulatory (β) subunits, is responsible for the phosphorylation of a large number of proteins and is implicated in cell division. Genomic clones coding for the CK2α subunit of Xenopus laevis have been isolated. Initial […]

Wilhelm V., Neckelman G., Allende J. E. and Allende C. C. The genomic structure of two Protein Kinase CK2α genes of Xenopus laevis and features of the putative promoter region. Mol. Cell. Biochem. 227, 175-183 (2001)
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Molecular dynamics study of the archaeal aquaporin AqpM

ABSTRACT: Aquaporins are a large family of transmembrane channel proteins that are present throughout all domains of life and are implicated in human disorders. These channels, allow the passive but selective movement of water and other small neutral solutes across cell membranes. Aquaporins have been classified into two subfamilies: i) strict aquaporins that only allow […]

Araya-Secchi R., Garate J. A., Holmes D. S. and Perez-Acle T. Molecular dynamics study of the archaeal aquaporin AqpM. BMC Genomics  11-13 (2001)
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Purification and characterization of a human factor that assembles and remodels chromatin

ABSTRACT: We have previously reported the isolation and characterization of a nucleosome remodeling and spacing factor, RSF. One of the RSF subunits is hSNF2h, a SNF2 homologue. Here we set out to isolate and characterize other hSNF2h-containing complexes. We have identified a novel hSNF2h complex that facilitates ATP-dependent chromatin assembly with the histone chaperone NAP-1. The complex possesses ATPase activity that is […]

LeRoy G., Loyola A., Lane W. S., Reinberg D. Purification and characterization of a human factor that assembles and remodels chromatin. J. Biol. Chem. 275(20): 14787–14790 (2000)
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For the full list of publications, visit Dr. Holmes’ Google Scholar profile: https://scholar.google.cl/citations?user=pKM2MPYAAAAJ&hl=en

For the full list of publications, visit Dr. Holmes’ Google Scholar profile: https://scholar.google.cl/citations?user=pKM2MPYAAAAJ&hl=en
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Use of a novel Hepatitis C major epitope chimeric polypeptide for the diagnosis of HCV viral infection.

ABSTRACT: The genome of hepatitis C virus (HCV) consists of seven functional regions: the core, E1, E2/NS1, NS2, NS3, NS4, and NS5 regions. The U. S. Food and Drug Administration-licensed 2.0G immunoassay for the detection of anti-HCV uses proteins from the core, NS3, and NS4 regions (McHutchinson et al., Hepatology 15:19-25, 1992). The 3.0G enzyme-linked […]

Chien D., Arcangel P., Medina M.A., Coit D., Baumeister M., Nguyen S., George-Nascimento C., Gyenes A., Kuo G. and Valenzuela P. Use of a novel Hepatitis C major epitope chimeric polypeptide for the diagnosis of HCV viral infection. J. Clin. Microbiol., 37 (5), 1393-1397 (1999).
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Peroxisome Proliferators and Signal Transduction

ABSTRACT: Hypolipidemic peroxisome proliferators (HPPS), as plasticizers, agrochemicals, Hypolipidemic drugs, and other structurally unrelated xenobiotics are known to induce a pleiotropy of similar short- and long-term effects in treated animals. Increased synthesis of specific mRNAs for peroxisomal and microsomal enzymes, which is followed by an increased number and size of hepatic peroxisomes and induction of specific […]

Bronfman M., Nuñez L., Orellana A., Burzio V. and Hidalgo P. Peroxisome Proliferators and Signal Transduction. ANN N. Y. Acad. Sci. 27 (804): 403-412 (1996)
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In sea urchin embryos the translation of cleavage stage histone variants is confined to S phase and is partially uncoupled from DNA replication

Imschenetzky M., Wilhelm V., Puchi M. and Medina R. In sea urchin embryos the translation of cleavage stage histone variants is confined to S phase and is partially uncoupled from DNA replication. Biol. Res. 28, 131-140 (1995)
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Expression of the subunits of protein kinase CK2 during oogenesis in Xenopus laevis

ABSTRACT: Northern-blot analysis of RNAs from different tissues demonstrated that the mRNA for the protein kinase CK2α subunit is very abundant in the ovary of Xenopus laevis. The competitive reverse-PCR technique has been used to quantitate the mRNA for both CK2α and CK2β subunits during oogenesis. The results obtained using eight different animals consistently show […]

Wilhelm V., Rojas P., Gatica M., Allende C. C. and Allende J. E. Expression of the subunits of protein kinase CK2 during oogenesis in Xenopus laevis. Eur. J. Biochem. 232, 671–676 (1995)
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Alpha-factor directed synthesis and secretion of mature foreign proteins in S. cerevisiae.

ABSTRACT: Saccharomyces cerevisiae cells were transformed with plasmids containing hybrid genes in which the sequence encoding mature human epidermal growth factor was joined to sequences encoding the leader region (preprosegment) of the precursor of the yeast mating pheromone alpha-factor. These cells accurately process the hybrid protein and efficiently secrete authentic biologically active human epidermal growth […]

Brake A.J., Merryweather J.P., Coit D.G., Heberlein U.A., Masiarz F.R., Mullenbach G.T., Urdea M.S., Valenzuela P., & BarrP.J. Alpha-factor directed synthesis and secretion of mature foreign proteins in S. cerevisiae. Proc. Natl. Acad. Sci. USA 8l, 4642-4646 (1984).
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Synthesis and assembly of hepatitis B virus surface antigen particles yeast

ABSTRACT: The surface antigen of hepatitis B virus (HBsAg) has been synthesized in the yeast Saccharomyces cerevisiae by using an expression vector that employs the 5′-flanking region of yeast alcohol dehydrogenase I as a promoter to transcribe surface antigen coding sequences. The protein synthesized in yeast is assembled into particles having properties similar to the […]

Valenzuela P., Medina A., Rutter W.J., Ammerer G. & Hall B.D. Synthesis and assembly of hepatitis B virus surface antigen particles yeast. Nature 298, 347 (1982)..
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Nucleotide sequence of the gene coding for the major protein of hepatitis B surface antigen.

ABSTRACT: DNA extracted from hepatitis B virus Dane particles has been cloned in bacteria using a plasmid vector. A full-length clone has been examined by restriction endonuclease analysis, and the nucleotide sequence of an 892-base pair fragment from cloned hepatitis B viral DNA encoding the surface antigen gene is reported. The amino acid sequence deduced […]

Valenzuela P., Gray P., Quiroga M., Zaldivar J., Goodman H.M. & Rutter W.J. Nucleotide sequence of the gene coding for the major protein of hepatitis B surface antigen. Nature 280, 215 (1979).
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Structure and processing yeast precursor tRNAs containing intervening sequences

ABSTRACT: We have isolated a precursor of yeast tRNATyr and shown that it contains an intervening sequence identical to that found in the gene for tRNATyr. The conformation of pre-tRNATyr is similar to that of mature tRNATyr except for the anticodon loop. The loop is sensitive to endonucleolytic cleavage by S1 nuclease near to the […]

O’Farrell P.Z., Cordell, B., Valenzuela P., Rutter W.J. & Goodman H.M. Structure and processing yeast precursor tRNAs containing intervening sequences Nature 274, 438-445 (1978).
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