PROFILE
In eukaryotes, DNA is compacted into chromatin, of which the basic unit is the nucleosome. Nucleosomes are comprised of two copies of each of four proteins histones wrapped by 147 base pairs of DNA. Compacting DNA allows the molecule to fit into cells in an orderly fashion, but makes DNA less accessible to cellular machinery such as transcription factors. To overcome this difficulty, histone post-translational modifications and histone variants maintain chromatin in a dynamic state regulating its accessibility. Thus, genes that are required for cellular processes can be expressed or repressed at the appropriate time. Our research focuses on understanding the mechanisms by which chromatin and its modifications regulate cellular processes. We have three main areas of investigation: the analysis of post-translational modifications of histones before they are incorporated in the chromatin, the role of the viral chromatin in the replication of the Hepatitis B Virus, and the characterization of the epigenetic modifications during the differentiation of T lymphocytes.
LAB MEMBERS
Research Head: Alejandra Loyola
Research Assistants: Tatiana Cruces • Claudia Espinoza • Danitza Silva
Post-doctoral Fellows: Francisco Saavedra • Simon Beard • Jose Manuel Jimenez
COLLABORATIONS
National Collaborations:
• Dr. Iván Alfaro (Universidad del Desarrollo)
• Dra. Andrea Calixto (Universidad Mayor)
• Dr. Rodrigo Pacheco (Fundación Ciencia & Vida)
• Dra. Raquel Quatrini (Fundación Ciencia & Vida)
• Dr. Mario Rosemblatt (Fundación Ciencia & Vida)
• Dr. Rodrigo Villanueva (Fundación Ciencia & Vida)
International Collaborations:
• Dr. Genevieve Almouzni (Institut Curie, Paris, France)
• Dr. Eric Campos (University of Toronto, Toronto, Canada)
• Dra. Thelma Escobar (Washington University)
• Dr. Axel Imhof (Ludwig-Maximilians Universität München, Munich, Germany)
• Dr. Danny Reinberg (Howard Hughes Medical Institute / NYU Langone School of Medicine, New York, USA)
FORMER LAB MEMBERS
Juan Hormazábal (Former Ph.D. student, 2016-2020)
Pilar Castillo (Former undergraduate student, 2015-2019)
Rodrigo Flores (Former post-doctoral fellow, 2018-2019)
Francisca Álvarez Astudillo (Former undergraduate student and Ph.D. student, 2009-2019)
Post-doctoral fellow at Universidad San Sebastian, Chile
Pía Vidal (Former post-doctoral fellow, 2018-2019)
Assistant professor at Universidad Catolica de la Santisima Concepcion
Francisca Muñoz Garrido (Former undergraduate student and Ph.D. student, 2009-2018)
Sebastián Marty Lombardi (Former undergraduate student, 2015-2017)
AG Siemens, Pharmakologisches Institut. Universität Heidelberg
Daniel Garrido Norambuena (Former Master degree student 2014-2017)
Biochemist at Laboratorio Quimica Clínica Especializada.
Sergio Hernández Tapia (Former Ph.D. student, 2013-2017)
Post-doctoral fellow at University of Marseille
Jocelyn Neira (Former Ph.D. student 2013-2017)
Head of the Flow Cytometer Facility at UNAB
Cristian Doñas Cuadra (Former undergraduate student and Ph.D. student, 2012-2017)
Post-doctoral fellow at Karolinska Institutet, Sweden
Lorena Rubio Quiroz (Former Master degree student 2013-2016)
Paola Merino (Former post-doctoral fellow, 2015)
Post-doctoral fellow at Emory University
Yvo Flores León (Former Master degree student 2013-2015)
Research assistant at INIA
Carlos Rivera Alvarez (Former Master degree student 2013-2015)
Ph.D. student at Pontificia Universidad Católica de Chile/Massachusetts General Hospital, Harvard Medical School
Germán Púas Villega (Former undergraduate student, 2012-2014)
Master of Philosophy, Universidad Santiago de Chile
Macarena Fritz Kelly (Former Ph.D. student, 2009-2013)
César Díaz Célis (Former post-doctoral fellow, 2012)
Post-doctoral fellow at HHMI/University of California
Valentina Ugalde Tagle (Former Master degree student 2009-2011)
Research assistant at Fundacion Ciencia & Vida