Abstract: Methods for DNA assembly have been instrumental in our pursuit of understanding the genetic code and our capacity to engineer biological functions. Among these, standardized Type IIS restriction endonuclease-based assembly systems have been adopted in different fields of biology due to their high efficiency and versatility, along with their amenability for using pre-compiled libraries of useful DNA components. We have developed Universal Loop assembly (uLoop), a standardised Type IIS assembly system for open, efficient and species-agnostic DNA fabrication, and we show its use by multi-spectral fluorescent protein expression in the diatom Phaeodactylum tricornutum, the yeast Saccharomyces cerevisiae, protoplasts of Arabidopsis thaliana and Escherichia coli.
Bernardo Pollak, PhD
Young Investigator – Millenium Institute for Integrative Biology (iBio)
Postdoctoral Fellow – Fundación Ciencia & Vida